Energy migration within hexameric hemoprotein reconstituted with Zn porphyrinoid molecules.
نویسندگان
چکیده
Photosensitizers, Zn protoporphyrin IX and Zn chlorin e6, are completely inserted into each heme pocket of a hexameric apohemoprotein. The fluorescence quenching efficiencies upon addition of methyl viologen are 2.3 and 2.6 fold-higher than those of the partially photosensitizer-inserted proteins, respectively, indicating that the energy migration occurs within the proteins.
منابع مشابه
Cross-Linking study of cytochrome P450 1A2 in proteoliposomes.
Proteoliposomes, containing cytochrome P450 1A2, were obtained by the cholate-dialysis technique. The effect of bifunctional cross-linking reagents on the purified hexameric cytochrome P450 1A2 in an aqueous medium and on the proteoliposomal P450 1A2 have been compared. Electrophoretic analysis of the modified proteins demonstrated the same oligomeric (hexameric) organization of the hemoprotein...
متن کاملElectron Transport Components of Hepatic Microsomes
Components of the hepatic microsomal P-450 hemoprotein electron transfer system (TPNH-cytochrome c reductase and P-450 hemoprotein) and the microsomal cytochrome b5 electron transfer system (DPNH-cytochrome bs reductase and cytochrome bS) were solubilized using Triton N-101 and glycerol, and separated using DEAE-cellulose chromatography. Microsomal aniline hydroxylase activity was reconstituted...
متن کاملCavity Waters Govern Insulin Association and Release: Inferences from Experimental Data and Molecular Dynamics Simulations
While a monomer of the ubiquitous hormone insulin is the biologically active form in the human body, its hexameric assembly acts as an efficient storage unit. However, the role of water molecules in the structure, stability and dynamics of the insulin hexamer is poorly understood. Here we combine experimental data with molecular dynamics simulations to investigate the shape, structure and stabi...
متن کاملA carbazole-containing porphyrinoid: synthesis and oxidation to the porphyrin-state.
Carbazole-containing porphyrinoid was synthesised for the first time via Suzuki-Miyaura cross-coupling reaction. Oxidation with MnO(2) yielded its porphyrin-state featuring macrocyclic aromaticity in exchange for the loss of the resonance energies of the benzene rings.
متن کاملMonitoring fluorescence of individual chromophores in peridinin-chlorophyll-protein complex using single molecule spectroscopy.
Single molecule spectroscopy experiments are reported for native peridinin-chlorophyll a-protein (PCP) complexes, and three reconstituted light-harvesting systems, where an N-terminal construct of native PCP from Amphidinium carterae has been reconstituted with chlorophyll (Chl) mixtures: with Chl a, with Chl b and with both Chl a and Chl b. Using laser excitation into peridinin (Per) absorptio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 55 شماره
صفحات -
تاریخ انتشار 2015